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. 2016 May;22(5):657–659. doi: 10.1261/rna.056325.116

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© 2016 Garcia and Parker; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Decay fragments are detectable in mTAG mRNAs when the MCP-GFP plasmid is present. Total RNA was isolated from yeast strains harboring MS2-tagged mRNAs and visualized by Northern blotting using a 1.5% formaldehyde agarose gel. Right lane of each blot is total RNA isolated from strains transformed with pUG36-CP-GFP×3, while the left lane is obtained from untransformed strains. The mRNAs analyzed on the same blot are grouped together. Visualization of the MS2-tagged mRNAs and decay products was done using the 12×MS2 probe (5′-CTGCAGACATGGGTGATCCTCATGTTTTCT-3′). Strains were grown to OD600 0.6 to 0.8 in minimal media (−MCP) or –ura minimal media (+MCP) at 30°C. (*) Indicates background bands.