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. 2016 May;22(5):773–781. doi: 10.1261/rna.055699.115

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© 2016 Grudzien-Nogalska et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Nudt3 is involved in reducing MCF-7 cell migration. (A) Immunofluorescence of filamentous (F)-actin (red) and DNA (blue) in proliferating Con^KD and Nudt3^KD MCF-7 cells. (B) Motility of Con^KD or Nudt3^KD MCF-7 cells complemented with the indicated proteins were analyzed by an in vitro scratch-wound healing assay. The cells were cultured to confluent cell monolayers, treated with mitomycin C to inhibit cell division, scratched and photographed at 0, 8, and 24 h after wounding. (C) Proliferation of Con^KD and Nudt3^KD MCF-7 cells was quantified by counting the number of cells at day 1, 2, 3, and 4 after initial seeding of 1 × 10⁵ cells per 35-mm dish. Data are representative of three independent experiments. (D,E) The percentage of gap closure for Nudt3^KD and Nudt4^KD cells were quantitated with ImageJ software. At least five different wounds were quantified for each time point. Data are representative of three independent experiments ±SD. P-values are denoted by asterisks. (***) P < 0.001 (Student's t-test).