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. 2016 Apr 19;11(4):e0153223. doi: 10.1371/journal.pone.0153223

Fig 6. NK cell depletion in EM/IOE-infected mice impairs expansion of effector memory CD8+ T cells.

Fig 6

Splenocytes harvested on day 7 after IOE infection from NK-/-EM/IOE and NK+/+EM/IOE-mice were stimulated in vitro with IOE antigens and the frequency of antigen-specific activated/effector memory T cells were determined by flow cytometry. Naïve cells were stimulated in vitro with IOE antigens. Gating on splenocytes and T cells was shown in (A). The spleens of NK-/-EM/IOE-mice contained lower percentages (B) and absolute number (C) of CD44+ CD8+ T cells compared to that detected in the spleens of NK+/+EM/IOE-infected mice. No significant difference in the frequency of CD62L+ CD8+ T cells between NK-/-EM/IOE-mice and NK+/+EM/IOE-mice. Data are presented as the means ± SD of four mice per group from two independent experiments. ** indicates P < 0.01.