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. 2016 Apr 19;11(4):e0153223. doi: 10.1371/journal.pone.0153223

Fig 9. Depletion of NK cells in NK-/-EM/IOE mice decreased production of IFN-γ.

Fig 9

The levels of IFN-γ in bulk culture of splenocytes from the indicated mice groups, harvested on day 7 after IOE infection, and stimulated in vitro with either E. muris (A) or IOE (B) Ags, were measured by ELISA. The antigen-specific IFN-γ response was calculated by subtraction of the IFN-γ concentration produced by unstimulated cells from the Ags-stimulated cells. The data show a significantly lower production of E. muris- (A) and IOE- (B) specific IFN-γ by splenocytes from NK-/-EM/IOE mice compared with similarly-stimulated cells from NK+/+EM/IOE mice. The levels of IFN-γ in NK-/-EM/IOE-mice were similar to those detected in naïve mice infected with IOE. * and ** indicate P < 0.05 and P < 0.01, respectively. Data are representative of two independent experiments with four mice per group.