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. 2016 Apr 19;11(4):e0153223. doi: 10.1371/journal.pone.0153223

Fig 10. Depletion of NK cells in NK-/-EM/IOE mice decreased production of iNOS and NO.

Fig 10

Spleen cells were harvested from the indicated mice groups on day 7 after IOE infection, and were stimulated in vitro with E. muris Ags or left unstimulated. (A) Dot plots shows negative control (cells stained with isotype control Ab) and positive control (splenocytes from IOE-infected mice stimulated in vitro with LPS). Cells were gated on leukocytes based on forward and side scatter. (B and C) Data show lower percentages and absolute number, respectively, of iNOS-producing leukocytes compared with NK+/+EM/IOE mice. (D) The level of NO produced by E. muris-stimulated cells was measured as described in Materials and Methods. Data show a significantly lower production of NO by splenocytes from NK-/-EM/IOE mice compared with NK+/+EM/IOE mice. The levels of NO in NK-/-EM/IOE-mice were similar to those detected in naïve mice infected with IOE. * and ** indicate P < 0.05 and P < 0.01, respectively. Data are representative of two independent experiments with four mice per group.