Fig 2. Reduced PrPC solubility is an effect specific to the binding of certain metal ions.
Protein suspensions from brain homogenates derived from C57BL wild-type mice were reacted with increasing concentrations of ZnCl2, CuCl2 and MgCl2 as indicated. Proteins were centrifuged resulting in a separation of PrPC into a fraction of high solubility in the supernatant and a fraction of low solubility in the pellet. Following SDS-PAGE and immunoblotting, PrPC signals were visualized using mabs SAF34 (A) and SAF70 (B) followed by chemiluminescence substrate development. PrPC specific bands are indicated with d (diglycosylated), m (monoglycosylated), n (nonglycosylated) and C1 (truncated glycosylated C1 fragment). Neuron-specific enolase (NSE) was used as control (C).