Fig 4. Reduced solubility due to ZnCl2 and CuCl2 is reversed by EDTA and SDS application.
Pooled brain tissues derived from C57BL wild-type mice (A) and bovine (B) homogenized in TBS and 2% N-octyl-β-D-glucopyranoside were supplemented with ZnCl2 (1 mM) and CuCl2 (1 mM) followed by incubation in the absence (0) or presence of EDTA in concentrations indicated or 1% SDS. Following centrifugation, proteins were separated into fractions of high solubility in the supernatant and low solubility in the pellet. PrPC signals were detected using mab SAF34 and visualized by chemiluminescence substrate development.