Fig 4. Alh represses Or47b and fru in developmentally related ORNs in the same sensillum during development.

(A) Asymmetric divisions of neuronal precursors give rise to ORNs in at4 sensilla. From the mutant phenotype, we predict that Alh represses Or47b and fru expression in Or88a and Or65a ORNs. (B) Alh ^GAL4-driven UAS-CD8GFP expression in developing pupal antennae. (C) Double labeling of Or47b (magenta) and alh (green) around 60–70 h APF (left panel) and 80 h APF. Or47b expression is excluded from alh expressing cells (arrows) but clusters with them (circled in white). (D) High magnification of a sensillum double labeled with Or47b-CD2 (magenta) and alh (green). Arrow points to the dendrite of Or47b ORN innervating the sensory hair together with alh-positive fibers. (E) Enlarged portions of wild-type (left panels) and alh mutant (right panels) antennae at 60–70 h APF, expressing Or47b-Gal4 UASCD8GFP (top panels), or fru-gal4 UASCD8GFP (bottom panels). (F) Wild-type (top panels) and alh mutant (bottom panels) antennal lobes expressing Or47bGal4UASCD8GFP (green) and stained for ncadherin (magenta).

GENOTYPES:

(B) 50hrs: AlhGal4 ^NP7010/UAS-CD8GFP; 60hrs-adult: AlhGal4 ^NP7441/UAS-CD8GFP

(C) Or47b-lexA lexOp-tomato:nls AlhGal4 ^NP6628/UAS-CD8GFP

(D) Or47B-CD2/+; AlhGal4 ^NP6628/UAS-CD8GFP

(E) eyFLP/+; Or47b-GAL4 UAS-CD8GFP/+; FRT82/FRT82Gal80E2F

eyFLP/+; Or47b-GAL4 UAS-CD8GFP/+; FRT82 alh¹³⁵³ /FRT82Gal80E2F

or eyFLP/+; UAS-CD8GFP/+; FRT82 fru ^GAL4 /FRT82Gal80E2F

eyFLP/+; UAS-CD8GFP/+; FRT82 alh¹³⁵³ fru^GAL4/FRT82Gal80E2F

(F) eyFLP/+; Or47b-GAL4 UAS-CD8GFP/+; FRT82 /FRT82Gal80E2F

or eyFLP/+; Or47b-GAL4 UAS-CD8GFP/+; FRT82 alh ¹³⁵³ /FRT82Gal80E2F