Fig 6. Or and Ir function is required to regulate fru expression in the adult olfactory system.

(A) Heterozygous orco mutant antennae (3–5 d old) expressing fruGal4 40XUASCD8GFP (A) and OR47b-CD2 (A’). (A”) shows the merge of two images. (B) Homozygous orco mutant antennae (3–5 d old). (C) Overexpression of UAS-orco under the control of fruGal4 in orco mutants (14 d old). (D) Homozygous Ir84a mutant antennae (3–5 d old) expressing fruGal4 40XUASCD8GFP. (E) Homozygous Ir8a mutant antennae (3–5 d old) expressing fruGal4 40XUASCD8GFP. (F) Heterozygous Ir84a mutant antennae (3–5 d old) expressing fruGal4 40XUASCD8GFP and orco-Gal80. (G) Heterozygous Ir8a mutant antennae (3–5 d old) expressing fruGal4 40XUASCD8GFP and orco-Gal80. (H) Quantification and statistical analysis of fru-positive ORN cell bodies observed in adult antennae of the indicated genotypes. n = 5–27. For all graphs, asterisks indicate significant (p < .005) differences from or47b heterozygotes. Error bars represent SEM. A one-way ANOVA was performed and followed with Tukey’s HSD—see Materials and Methods. All count data may be found in the Supporting Information as S1 Data.

GENOTYPES:

A–A”: Or47b-CD2 /+;orco ¹ fru ^GAL4 UAS-40XCD8GFP /+

B–B”: Or47b-CD2 /+;orco ¹ fru ^GAL4 UAS-40XCD8GFP /orco ¹

C: +/ UAS-orco; orco ¹ fru ^GAL4 UAS-40XCD8GFP /orco ¹

D: Ir84a ^MI00501 fru ^GAL4 UAS-40XCD8GFP / Ir84a ^MI00501

E: Ir8a ¹ /Y; fru ^GAL4 UAS-40XCD8GFP

F: orco-GAL80/+; Ir84a ^MI00501 fru ^GAL4 UAS-40XCD8GFP / +

G: orco-GAL80/+; Ir84a ^MI00501 fru ^GAL4 UAS-40XCD8GFP / Ir84a ^MI00501