Table 1. Primer and probes used for viral detection.
Primer or probe | Sequence (5’to 3’) | Polarity | References | |
---|---|---|---|---|
VP2-F1 | TCTGCAGACAGTTGAACCTATTAA | + | ||
Rotavirus | VP2-F2a | CAGACACGGTTGAACCCATTAA | + | [23] |
VP2-F3a | TCGGCTGATACAGTAGAACCTATAAATG | + | ||
VP2-F4a | TGTCAGCTGATACAGTAGAACCTATAAATG | + | ||
VP2-F5a | TCAGCTGACACAGTAGAACCTATAAATG | + | ||
VP2-R1a | GTTGGCGTTTACAGTTCGTTCAT | - | ||
VP2-R2a | GTTGGCGTCTACAATTCGTTCAT | - | ||
VP2-Pb | 6FAM-ATGCGCATRTTRTCAAAHGCAA | ++ | ||
Norovirus Génogroupe I | JJV1NF a: | CCA TGT TCC GTT GGA TGC | + | [24] |
JJV1R a: | TCC TTA GAC GCC ATC ATC AT | - | ||
JJV1P b: | FAM-5’-TGT GGA CAG GAG ATC GCA ATC TC -3’-TAMRA | + | ||
RING-1b b : | FAM-5’-AGA TCG CGG TCT CCT GTC CA-3’-TAMRA | - | ||
Norovirus Génogroupe II | QNIF2d a : | ATG TTC AGR TGG ATG AGR TTC TCW GA | + | [25] |
COG2Ra : | TCG ACG CCA TCT TCA TTC ACA | - | ||
QNIFS b : | FAM-5’-AGC ACG TGG GAG GGC GAT CG -3’-TAMRA | + | ||
Astrovirus | AV1TRa | CCG AGT AGG ATC GAG GGT | - | [26] |
AV2TRa | GCT TCT GAT TAA ATC AAT TTT AA | + | ||
AVsb | FAM-5'-CTT TTC TGT CTC TGT TTA GAT TAT TTT AAT CAC C-3'-TAMRA | |||
Sapovirus | SaV124Fa | GAY CAS GCT CTC GCY ACC TAC | + | [27] |
SaV1Faa | TTG GCC CTC GCC ACC TAC | + | ||
SaV1245Rb | CCC TCC ATY TCA AAC ACT A | - | ||
SaV124TPb | FAM-CCR CCT ATR AAC CA-MGB | - | ||
Aichivirus A | AICHI 6261a | ACA CTC CCA CCT CCC GCC AGT A | + | [28] |
AICHI 6779a | GGA AGA GCT GGG TGT CAA GA | - |
a Primer
b Probe