Fig 4. Time-dependent decrease in supplemented L. plantarum in Chd1^-/- but not Chd1^WT/WT flies.

Flies were fed on L. plantarum overnight, transferred to fresh food vials and collected at the indicated days after inoculation. (A) Lactobacillus load was determined by plating fly homogenates on MRS agar; (B) L. plantarum was detected by real-time PCR. Signals were normalized against the Drosophila GAPDH gene and mean values ±SEM of three biological replicates are shown. Significant differences between the two fly lines were analyzed by t-test (level of significance set to p<0.05) and are marked by (*).