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. 2016 Apr 19;11(4):e0153517. doi: 10.1371/journal.pone.0153517

Fig 1. Sequence characterization of RAV family members of Thellungiella salsuginea and Arabidopsis thaliana.

Fig 1

(A) Phylogenetic tree of the RAV family members in Thellungiella salsuginea and Arabidopsis thaliana. The phylogenetic tree was constructed using full-length protein sequences by the maximum-likelihood method with MEGA 5.0 and a bootstrap value of 1,000. The two major phylogenetic clades are designated as groups A and B. Shown on the right are diagrams of RAV proteins with information on the structure and position of different protein domains. (B) RAV subfamily-specific amino acids and their locations along the RAV full-length sequences. The amino acid sequences in boxes represent the conserved AP2 and B3 DNA-binding domains, which are characteristic of RAV transcription factors. The locations of the conserved YRG and RAYD elements are indicated as well. (C) Schematic illustrations of the types and distributions of motifs for each TsRAV family members with a same group. Motifs were identified using the MEME search tool and numerically marked according to their statistical significance (low E-value) in a descending order.