Figure 2. The effect of APOE haplotypes on human-APOE mRNAs expression levels in humanized mice brain tissues.

RNA was extracted from whole brain of three mice homozygotes for the human APOEe3 and three mice APOEe4 homozygous generated by targeted replacement²⁸. Fold levels of human APOE mRNA were assayed in whole brain tissues by real-time RT-PCR using TaqMan technology and calculated relative the geometric mean of the mouse housekeeping genes, Gapdh- and Ppia-mRNAs reference control using the 2^−ΔΔCt method. The expression levels between e4/4 and e3/3 were compared and the values presented here are means levels ± SE.