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. Author manuscript; available in PMC: 2016 Apr 19.
Published in final edited form as: Methods Cell Biol. 2015 Jul 7;130:347–367. doi: 10.1016/bs.mcb.2015.05.008

FIGURE 3. Internalization of 125I-EGF.

FIGURE 3

(A) NIH 3T3/EGFR cells were incubated with 1 ng/mL 125I-EGF for 1–6 min at 37 °C, and the amount of surface and internalized radioactivity was determined as described in Section 4.2. (B) UMSCC2 cells were incubated with 30 µM Dyngo-4a in DMEM, and the internalization assay with 1 ng/mL 125I-EGF was performed as in (A).