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. Author manuscript; available in PMC: 2016 Apr 19.
Published in final edited form as: J Phys Chem B. 2015 Dec 4;119(50):15443–15450. doi: 10.1021/acs.jpcb.5b10136

Figure 3.

Figure 3

Effect of aggregation reducing mutations (V74E and T72P). (a) Reaction-limited rates. The dashes represent the lower limit of this rate. (b) (circles) Diffusion-limited rates, normalized to the viscosity of water at that temperature. Error bars are determined from the linear fits. (triangles) The observed rates are diffusion limited (kobs ≈ kD+), and the error bars are the standard deviation of multiple measurements. The subscripts in the legend indicate the location of the Trp and Cys. (c) Average root-mean-square distance between the Trp and Cys determined for each reaction-limited rate using eqs 4 and 6. Error bars are determined as the change in distance required to change kR by the measured error. (d) Intramolecular diffusion coefficients determined for each diffusion-limited rate using eq 5. The error bars are determined as proportional to the error in kD+. (triangles) D determined from values in (b) using eq 3 and ⟨r2⟩ = 400 ± 100 Å2. The error bars are the propagated error of kD+ and ⟨r2⟩.