Fig. 7.
Analysis of lacA transcription in a LacR-2 operator mutant in pneumococcus. lacA transcription during mid-exponential growth of was assessed by qRT-PCR in WT, ΔlacR-2 and the PlacA* strain, which has a three-base pair substitution (TGT→ACA) in this putative LacR-2 operator. Cells were grown to mid-exponential phase in CDM with the following carbohydrates: 0.5% glucose (white bars), galactose (gray bars) or 0.5% glucose and 0.5% galactose (gray and white striped bars), the latter condition representing a carbon catabolite-repressive condition. Asterisk indicates a statistically significant difference in expression compared to the corresponding WT sample (P value ≤ 0.05)