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. Author manuscript; available in PMC: 2016 Sep 21.
Published in final edited form as: Nat Immunol. 2016 Mar 21;17(5):505–513. doi: 10.1038/ni.3400

Figure 2. Loss of butyrate decreases histone acetylation in IECs.

Figure 2

(a) Protein expression of acetyl-histone H4 (top blot) and densitometric analysis normalized to presence of α-tubulin (shown right of blot) 21 days following syngeneic (BALB/c → BALB/c) or allogeneic (C57BL/6J → BALB/c) BMT. Densitometric analysis of 3 experiments combined; each experiment had n=5-6 mice per group. (b) Gene expression of representatives of class I, II, and IV HDAC enzymes, (c) histone acetyltransferase (HAT) enzyme levels, (d) HDAC activity, and (e) HAT activity in syngeneic and allogeneic CD326+ IECs. A = absorbance. (f) Gene expression and (g) protein levels of SLC5A8 (monocarboxylate transporter of butyrate) in IECs (CD326+) of syngeneic and allogeneic transplant recipients 21 days following BMT. Representative immunoblots; densitometric analysis of three similar experiments combined is shown right of blots. *P < .05; **P < .01 of students t-test. Bars and error bars represent the means and standard errors of the mean, respectively.