Figure 1b:
(a) Photomicrographs (original magnification, ×20) show the localization of human adipose tissue–derived MSCs. GFP was used to stably transfect 2.5 × 105 MSCs, which were injected into the adventitia of the outflow vein of the AVF at the time of creation. GFP-labeled human adipose tissue–derived MSCs are present on day 7 in vessels with transplanted MSCs compared with outflow vein vessels removed from control animals after AVF placement. Nuclei are blue. There are GFP-positive cells (arrows) in the vessel wall of the outflow vein on day 7. * = lumen. (b) Serial PET images of 89Zr distribution in mice after adventitial delivery of 89Zr-labeled MSCs or 89Zr (HPO4)2. The anatomic reference skeleton images are formed by using the mouse atlas registration system algorithm with information obtained from the stationary top-view planar x-ray projector and side-view optical camera. SUV = standardized uptake value.