Figure 4:

Photomicrographs (original magnification, ×100 [top row] and ×40 [bottom row]) show TUNEL and Ki-67 staining in murine AVF on day 7 after placement of AVF in the outflow vein alone (control animals) and in vessels with transplanted MSCs. The upper row is representative sections from TUNEL staining at the outflow vein of the vessels with transplanted MSCs and control vessels on day 7. Brown-staining nuclei are positive for TUNEL (arrows). The negative control is shown where the recombinant terminal deoxynucleotidyl transferase enzyme was omitted. The lower row is representative sections after Ki-67 staining in outflow vessels with transplanted MSCs or AVF alone in the control animals on day 7 after AVF placement. Brown-staining nuclei are positive for Ki-67. Negative controls stained with immunoglobulin G are shown.