miR-186 decreases both mRNA and protein expression levels of endogenous BACE1 in neuronal cells. (a) Reduction of Bace1 mRNA by miR-186 in Neuro-2a cells. 48 h post-transfection with 75 nM of negative control or miR-186, Bace1 mRNA levels were measured by qRT-PCR and normalized by corresponding Gapdh levels (n = 6, **, p<0.01; t-test). (b, c) Dose-dependent decrease of endogenous BACE1 protein levels by miR-186 in Neuro2a cells. 48 h post-transfection with indicated amount of negative control or miR-186, BACE1 protein levels were monitored by Western blot. Representative Western blot images (b) and relative BACE1 protein levels (c) are shown. BACE1 protein levels were normalize by corresponding β-actin levels (n = 4). All data are shown as a percentage of control. Values are mean ± SEM (**, p<0.01; ***, p<0.001, One-way ANOVA compared to control with post hoc Dunnett’s test).