Inhibition of endogenous miR-186 increases BACE1 expression in neuronal cells. (a, b) Inhibition of miR-186 increases BACE1 protein levels in Neuro-2a cells. Neuro-2a cells were transfected with 50 nM of anti-miR-186 or anti-control (anti-Ctl). 72 h post-transfection, cell lysates were applied for Western blot. A representative western blot images (a) and relative BACE1 levels (b) are shown. BACE1 protein levels were quantified as a percentage of control after normalization by corresponding β-actin levels (n = 7). (c) Inhibition of miR-186 does not affect BACE1 mRNA levels in Neuro-2a cells. Neuro-2a cells were transfected with 50 nM of anti-miR-186 or anti-control (anti-Ctl). 72 h post-transfection, Bace1 mRNA levels were measured by qRT-PCR and normalized by corresponding Gapdh levels (n = 7). Data are shown as a percentage of control. Values are mean ± SEM (***, p<0.001, t-test).