Table 2.
Summary of the PCR primers used in this study for speciation of gastric Helicobacter infection
| Identification | Gene | Designation | Direction | Amplicon size (bp) | Sequence (5′ to 3′) | Reference |
|---|---|---|---|---|---|---|
| Helicobacter spp. | 16S rRNA | C97 C05 |
Forward Reverse |
1200 | GCTATGACGGGTATCC ACTTCACCCCAGTCGCTG |
Fox et al. [11] |
| H. pylori | Urease C | Chp1 Chp2 |
Forward Reverse |
294 | AAGCTTTTAGGGGTGTTAGGGGTT AAGCTTACTTTCTAACACTAACGC |
Bickley et al. [12] |
| H. pylori | HSP60 | H60F H60R |
Forward Reverse |
600 | GGNGAYGGNCANCANCANGCNC TCNCCRAANCCNGGNGCYTTNCA |
Mikkonen et al. [51] |
| H. suis | 16S rRNA | V832f V1261r |
Forward Reverse |
433 | TTG GGA GGC TTT GTC TTT CCA GAT TAG CTC TGC CTC GCG GCT |
De Groote et al. [13] |