Table 4.
Molecular characterization of gastric Helicobacter species in animals 9 and 10
| Animal no. | Signalment | PCR
|
16s rRNA sequence
|
||||
|---|---|---|---|---|---|---|---|
| H. spp | H. suis | H. pylori | % Identity | BLAST Description | |||
| 9 | Female, 20 year 2 month | Gastric Wash | + | + | − | 99% | AF506793.1 - Helicobacter suis strain RM1 |
| Gastric Wash 2 | + | + | − | 95% | L36143.1 - Helicobacter pullorum NCTC 12826 | ||
| Gastric Wash 3 | + | + | − | 95% | L36143.1 - Helicobacter pullorum NCTC 12826 | ||
| Antrum | + | + | − | 95% | L36143.1 - Helicobacter pullorum NCTC 12826 | ||
| Fundus | + | + | − | 95% | L36143.1 - Helicobacter pullorum NCTC 12826 | ||
| 10 | Male, 21 year 10 month | Gastric Wash | + | − | − | ||
| Antrum | + | + | − | 97% | AF506793.1 - Helicobacter suis strain RM1 | ||
| Fundus | + | + | − | ||||
DNA isolated from fresh frozen gastric wash and tissue tested positive for Helicobacter spp. infection via PCR and confirmed with 16S rRNA sequence analysis. Novel Helicobacter species indicated by those matching closest (95%) homology to H. pullorum.