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. Author manuscript; available in PMC: 2016 Sep 28.
Published in final edited form as: Nat Chem Biol. 2016 Mar 28;12(5):367–372. doi: 10.1038/nchembio.2051

Figure 1.

Figure 1

Discovery and characterization of AIG1 and ADTRP as FP-reactive proteins in the human proteome. (a) Competitive ABPP-SILAC analysis to identify FP-alkyne-inhibited proteins, where protein enrichment and inhibition were measured in proteomic lysates from SKOV3 cells treated with FP-alkyne (20 μM, 1 h) or DMSO using the FP-biotin probe following established protocols14. Annotated Ser hydrolases are indicated by red markers; AIG1 is marked in black and with an arrow. Data represent the median SILAC ratios ± s. d. for peptides quantified for each protein from one experiment representative of two biological replicates. (b) Sequence alignment of AIG1 orthologues from multiple species, as well as homologous ADTRP proteins (http://www.st-va.ncbi.nlm.nih.gov/tools/cobalt/cobalt.cgi). Residues conserved between all sequences are marked with asterisks. The conserved Thr (Thr43 in human AIG1) and His (His134 in human AIG1) residues are boxed in red.