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. Author manuscript; available in PMC: 2016 Sep 28.
Published in final edited form as: Nat Chem Biol. 2016 Mar 28;12(5):367–372. doi: 10.1038/nchembio.2051

Figure 2.

Figure 2

Identification of Thr and His residues critical for FP labeling of AIG1 and ADTRP. (a-d) ABPP gels and Western blots of membrane proteomes (0.5 mg proteome mL−1) showing selective FP-Rh labeling of WT-, but not the indicated Thr-to-Ala and His-to-Ala mutants of human (h) AIG1 (a, c) and ADTRP (b, d) in transfected HEK293T cell lysates. Proteomes were treated with 1 μM FP-Rh for 30 min at 37 °C. Full images of gels and Western blots are provided in Supplementary Figure 16.