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Predicted novel miRNAs identified in this study. (A) Predicted stem-loop structures of novel miRNA precursors. The precursor structures of five newly identified rice miRNAs (novel-miR-259, 169, 368, 221, and 32) were predicted via the MFOLD pipeline. Mature miRNA sequences are highlighted in red, and miRNA169* is highlighted in blue. (B) Stem-loop RT-PCR analysis of the identified novel miRNAs. M indicates a 20-bp DNA Ladder Marker.
