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. 2016 Apr 20;7:509. doi: 10.3389/fmicb.2016.00509

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Copyright © 2016 Yamaoka, Matsuyama, Fukushi, Matsunaga, Matsushima, Kuroyama, Kimura, Takeda, Chimuro and Ryo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Epitope mapping of mAbs. (A) Schematic diagram of MERS-NP putative domain architecture and deletion mutants. For epitope mapping, 11 deletion mutants were produced as His-tagged proteins in the wheat germ cell-free system. NTD, N-terminal domain; LKR, flexible linker region; CTD, C-terminal domain. (B) Confirmation of protein expression. His-tagged NP and deletion mutants were immunoblotted with anti-His-tagged antibody. (C) Summary of epitope analysis. Reactivity of each mAb to deletion mutants was evaluated by immunoblotting. + and - indicate positive and negative detection, respectively. NT; not tested. Negative detections are highlighted in gray.