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. 2016 Apr 20;7:509. doi: 10.3389/fmicb.2016.00509

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Copyright © 2016 Yamaoka, Matsuyama, Fukushi, Matsunaga, Matsushima, Kuroyama, Kimura, Takeda, Chimuro and Ryo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Establishment of antigen detection assays for MERS-CoV. (A) Immunoblot analysis for virus particles of MERS-CoV. Cell culture medium of MERS-CoV-infected Vero-TMPRSS2 cells were collected and analyzed by immunoblotting with the newly developed anti-MERS-NP mAbs. (B,C) Detection limit of ELISA and ICT. Recombinant antigen protein and MERS-CoV were subjected to either ELISA or ICT analysis. + and - indicate positive and negative detection, respectively; ± means moderately positive. Arrowheads show positive test line.