Table 3.

Reaction time optimization for RT-LAMP^a.

Time/min	Primers group	RNA dilution (Copies/μl)
		107	106	105	104	103	102	101	100
30	1b	+	+	+d	+d	-	-	-	-
	2c	+	+	+	+	-	-	-	-
40	1	+	+	+	+	+	+	-	-
	2	+	+	+	+	-	-	-	-
50	1	+	+	+	+	+	-	-	-
	2	+	+	+	+	-	-	-	-
60	1	+	+	+	+d	-	-	-	-
	2	+	+	+	+	+	-	-	-

^aThree replications were performed for each trial. ^bThe six primers was used as described as bellow: 0.4 μM each of inner primers FIP and BIP, 0.2 μM loop primers LF and LB, 0.1 μM outer primers F3 and B3. ^cThe six primers was used as described as bellow: 0.2 μM each of inner primers FIP and BIP, 0.1 μM loop primers LF and LB, 0.05 μM outer primers F3 and B3. ^dThe result was weakly positive.