Fig. 7.

PfHsp70-z associates with PfHsp70-1. Pulldown assays conducted using antibodies against PfHsp70-z and recombinant PfHsp70-z protein. Prey proteins in parasite cell lysate harvested from cultures growing under normal temperature (37 °C) and heat stress (42 °C) conditions. Prey protein was allowed to bind onto columns on which the antibody (a) or recombinant PfHsp70-z (b) were immobilized, respectively. Binding was allowed to occur in the absence of nucleotide (NN) or presence of 5 μM ADP/ATP, respectively. The eluate was analyzed under reducing conditions and subsequently probed by Western blot using anti-PfHsp70-1 antibodies. The representation of the densitometric analyses for data is provided (lower panels; c and d, respectively). Lane C: control sample (conducted on column with beads minus anti-PfHsp70-z or PfHsp70-z recombinant protein). Cell lysate used in control assay was obtained from culture incubated at 37 °C. Lanes 37 and 42 °C: Immunoaffinity conducted using cell lysate obtained from cells cultured at the respective temperatures which was allowed to bind to beads onto which anti-PfHsp70-z (a) or recombinant PfHsp70-z (b) were immobilized, respectively. Arrows in panel a show bands representing PfHsp70-1 (70-1) and Ig heavy chain (Ig), respectively