Fig. 4.

Phosphorylation of HSF1 at Ser³²⁶ upon infection with H. pylori P12wt and P12ΔcagA mutant. Phosphorylation of HSF1 at Ser³²⁶ as well as HSF1 total levels within cell lysates of AGS infected with H. pylori P12wt or P12ΔcagA for 1, 3, 6, 12, and 24 h were examined by Western blot; representative blots are shown. Densitometric analysis of HSF1 p-Ser³²⁶/HSF1 mean expression ratio at 3-h p.i. (n = 2) and of HSF1/actin at 24-h p.i. (n = 4) is shown on right, ±SEM