Fig. 5.

Overexpression of HSF1 partially rescues HSP repression upon H. pylori infection of AGS cells. a AGS cells overexpressing HSF1WT, HSF1ΔRDT, and the GFP control cell line were inoculated with H. pylori P12wt (MOI:50) or BHI broth, and HSP levels were analyzed 24 h post-infection by Western blot, n = 3. Densitometric analysis of mean fold change in HSPD1 levels ±SEM are shown on right expressed as a ratio of that of the BHI control for each given cell line, *p < 0.05. b CagA-expression vectors were used to transiently express CagA-HA or PR-CagA-HA in AGS cells. Immunostaining of AGS cells ectopically expressing CagA-HA and PR-CagA-HA using anti-HA and anti-HSF1 antibodies revealed no co-localization of CagA with endogenous HSF1. Top two rows represent secondary antibody control and empty SRα expression vector controls, respectively, n = 2. Scale bar = 25 μm