Table 2.
Precision of retention times for histone H4-derived peptides after chymotrypsin and trypsin digestion analyzing six replicates spread over three different days
| Measured peptide forms | Average retention time (n = 18) | Relative standard deviation (%) |
|---|---|---|
| [+d0-/d6-]GK[+56.0]GGK[+56.0]GL | 17.688 | 0.028 |
| [+d0-/d6-]GK[+56.0]GGAK[+56.0]R | 13.610 | 0.000 |
| [+d0-/d6-]GK[+42.0]GGK[+56.0]GL | 16.848 | 0.010 |
| [+d0-/d6-]GK[+56.0]GGK[+42.0]GL | 17.077 | 0.044 |
| [+d0-/d6-]GK[+42.0]GGAK[+42.0]R | 12.781 | 0.096 |
| [+d0-/d6-]GK[+42.0]GGAK[+56.0]R | 13.176 | 0.039 |
| [+d0-/d6-]GK[+56.0]GGAK[+42.0]R | 13.180 | 0.000 |
| [+d0-/d6-]GK[+56.0]GGK[+56.0]GL | 17.694 | 0.045 |
| [+d0-/d6-]GK[+56.0]GGAK[+56.0]R | 13.611 | 0.014 |
| [+d0-/d6-]GK[+42.0]GGK[+56.0]GL | 16.853 | 0.034 |
| [+d0-/d6-]GK[+56.0]GGK[+42.0]GL | 17.091 | 0.265 |
| [+d0-/d6-]GK[+42.0]GGAK[+42.0]R | 12.780 | 0.117 |
| [+d0-/d6-]GK[+42.0]GGAK[+56.0]R | 13.167 | 0.101 |
| [+d0-/d6-]GK[+56.0]GGAK[+42.0]R | 13.181 | 0.015 |
The levels refer to the following (d0-/d6-) mixing ratios: 0.5:1 (upper part) and 0.3:1 (lower part)