Table 1.
List of primers for gene expression analysis.
| Gene | Accession number | Oligonucleotide sequence (5′ → 3′) |
|---|---|---|
| ACAN | NM_173981 | F: CCAACGAAACCTATGACGTGTACT |
| R: GCACTCGTTGGCTGCCTC | ||
| COL1 | NM_001034039 | F: TGAGAGAGGGGTTGTTGGAC |
| R: GGGAGACCATTGAGTCCATC | ||
| COL2 | NM_001113224 | F: TGGCTGACCTGACCTGAC |
| R: GGGCGTTTGACTCACTCC | ||
| MMP13 | NM_174389 | F: CCTTGATGCCATAACCAGTCTCC |
| R: ATCAATACGGTTGGGAAGTTCTGG | ||
| TIMP1 | NM_174471 | F: GTCAATGAAACTGCCTTATACC |
| R: TTCTGGGACCTGTGGAAG | ||
| TIMP2 | NM_174412 | F: GCAACGACATCTACGGCAACC |
| R: CCCACACACGGCAGAGGAG | ||
| COX2 | NM_174445 | F: TCCACCAACTTATAATGTGCAC |
| R: GGCAGTCATCAGGCACAGGA | ||
| GAPDH* | NM_001034034 | F: GGCGTGAACCACGAGAAGTATAA |
| R: CCCTCCACGATGCCAAAGT | ||
| 18s* | (Primer Design Ltd) |
* Reference gene
ACAN = aggrecan; COL1/COL2 = collagen type I/II; MMP13 = matrix metalloprotease 13; TIMP1/TIMP2 = tissue inhibitor of metalloproteinase 1/2; COX2 = cyclooxygenase 2; GAPDH = glyceraldehydes-3-phosphate dehydrogenase