Table 1.
Features of bacteriophage UAB_Phi20 genome, ORFs, gene products, and functional assignments.
| ORF | Gene | Position (nt) | Strand | No of amino acids | Predictive function | Closest hit (Accession number) | % Amino acid identity | Best e-value | |
|---|---|---|---|---|---|---|---|---|---|
| From | To | ||||||||
| 1 | 27 | 173 | + | 48 | Unknown | Hypothetical protein P22gp50(NP_059609.1) | 100 | 9.E-25 | |
| 2 | gp18 | 166 | 981 | + | 271 | DNA replication (primase) | Hypothetical protein P22gp51 (NP_059610.1) | 100 | 0 |
| 3 | gp12 | 978 | 2354 | + | 458 | DNA replication (helicase) | Hypothetical protein P22gp52 (NP_059611.1) | 100 | 0 |
| 4 | ninA | 2351 | 2431 | + | 26 | Unknown | Nin A [Enterobacteria phage P22](YP_063725.1) | 100 | 5.E-20 |
| 5 | ninB | 2428 | 2865 | + | 145 | Unknown | NinB [Enterobacteria phage P22] (NP_059612.1) | 99 | 6.E-101 |
| 6 | ninD | 2862 | 3035 | + | 57 | Unknown | Nin D [Enterobacteria phage P22] (YP_063726.1) | 100 | 7.E-35 |
| 7 | ninE | 3002 | 3178 | + | 58 | Unknown | Nin E [Enterobacteria phage P22] (NP_059614.1) | 100 | 6.E-34 |
| 8 | ninX | 3175 | 3513 | + | 112 | Unknown | Nin X [Enterobacteria phage P22] (NP_059615.1) | 100 | 1.E-77 |
| 9 | ninF | 3506 | 3682 | + | 58 | Unknown | Nin F [Enterobacteria phage P22] (YP_063727.1) | 100 | 2.E-33 |
| 10 | ninG | 3672 | 4286 | + | 203 | Unknown | Nin G [Enterobacteria phage P22] (YP_063728.1) | 100 | 6.E-145 |
| 11 | ninY | 4283 | 4507 | + | 74 | Unknown | Nin Y [Enterobacteria phage P22] (NP_059618.1) | 100 | 5.E-48 |
| 12 | ninH | 4504 | 4707 | + | 67 | Unknown | Nin H [Enterobacteria phage P22] (NP_059619.1) | 100 | 3.E-41 |
| 13 | ninZ | 4688 | 4867 | + | 59 | Unknown | Nin Z [Enterobacteria phage P22] (YP_063729.1) | 100 | 3.E-34 |
| 14 | 23 | 4864 | 5487 | + | 207 | Transcription antitermination protein | Gp63 [Enterobacteria phage P22] (YP_063730.1) | 100 | 9.E-153 |
| 15 | 5577 | 5786 | + | 69 | Unknown | Hypothetical protein ε34gp63 (YP_002533523.1) | 97 | 2.E-40 | |
| 16 | 5809 | 5913 | + | 34 | Unknown | ||||
| 17 | gp13 | 5922 | 6248 | + | 108 | Holin | Holin [Enterobacteria phage P22] (NP_059621.1) | 100 | 1.E-70 |
| 18 | gp19 | 6229 | 6669 | + | 146 | Lysozyme | Gp66 [Enterobacteria phage P22] (NP_059622.1) | 100 | 1.E-101 |
| 19 | gp15 | 6804 | 7103 | + | 99 | Endopeptidase Rz | Hypothetical protein P22gp67 (NP_059623.2) | 100 | 2.E-64 |
| 20 | Rz1 | 6838 | 7050 | + | 70 | Lipoprotein Rz1 precursor | Hypothetical protein P22gp68 (YP_063732.1) | 100 | 1.E-34 |
| 21 | 7144 | 7329 | + | 61 | Unknown | ||||
| 22 | Rha | 7322 | 7858 | + | 178 | Unknown | Rha [Enterobacteria phage P22] (NP_059624.1) | 100 | 9.E-130 |
| 23 | 7940 | 8296 | + | 118 | Unknown | Hypothetical protein SE1gp48 (YP_002455884) | 100 | 7.E-78 | |
| 24 | 8300 | 8689 | + | 129 | Unknown | Hypothetical protein ST64Tp49 (NP_720323.1) | 100 | 8.E-92 | |
| 25 | 8689 | 9093 | + | 134 | Unknown | Hypothetical protein ST64Tp50 (NP_720324.1) | 100 | 2.E-90 | |
| 26 | gp3 | 9097 | 9585 | + | 162 | Terminase (small subunit) | ST64Tp51 (NP_720325.1) | 100 | 3.E-116 |
| 27 | gp2 | 9737 | 11062 | + | 441 | Terminase (large subunit) | Gp2 [Enterobacteria phage ST104] (YP_006405) | 99 | 0 |
| 28 | gp1 | 11062 | 13239 | + | 725 | Portal protein | Gp1 [Salmonella enterica bacteriophage SE1] (YP_002455889.1) | 100 | 0 |
| 29 | gp8 | 13253 | 14164 | + | 303 | Scaffolding protein | Gp8 [Enterobacteria phage P22] (YP_063736.1) | 100 | 0 |
| 30 | gp5 | 14164 | 15456 | + | 430 | Coat protein | Coat protein [Enterobacteria phage ST64T] (NP_720329.1) | 99 | 0 |
| 31 | 15495 | 15704 | + | 69 | Unknown | Hypothetical protein P22gp06 (NP_059631.1) | 100 | 5.E-40 | |
| 32 | gp4 | 15688 | 16188 | + | 166 | DNA stabilization protein | Gp4[Enterobacteria phage P22] (NP_059632.1) | 100 | 8.E-120 |
| 33 | gp10 | 16148 | 17566 | + | 472 | Packaged DNA stabilization protein | Head completion protein [Enterobacteria phage P22] (NP_059633.1) | 100 | 0 |
| 34 | gp26 | 17570 | 18271 | + | 233 | Head completion protein | Gp26[Enterobacteria phage P22] (YP_063715.1) | 100 | 5.E-165 |
| 35 | gp14 | 18271 | 18726 | + | 151 | Head assembly protein | Gp14 [Enterobacteria phage P22] (YP_063716.1) | 100 | 6.E-109 |
| 36 | gp7 | 18729 | 19418 | + | 229 | Injection protein | Gp7 [Enterobacteria phage P22] (YP_063717.1) | 100 | 2.E-154 |
| 37 | gp20 | 19429 | 20844 | + | 471 | Injection protein | Gp20 [Enterobacteria phage P22] (NP_059637.1) | 100 | 0 |
| 38 | gp16 | 20844 | 22673 | + | 609 | Injection protein | Gp16 [Enterobacteria phage P22] (YP_063718.1) | 100 | 0 |
| 39 | sieA | 23406 | 22696 | − | 236 | Superinfection exclusion | SieA [Enterobacteria phage P22] (NP_059639.1) | 99 | 7.E-110 |
| 40 | hkcC | 23221 | 23586 | + | 121 | Unknown | hkcC [Bacteriophage HK620] (NP_112089.1) | 100 | 5.E-85 |
| 41 | 23794 | 23600 | − | 64 | Unknown | Hypothetical protein P22gp16 (NP_059640.1) | 98 | 5.E-33 | |
| 42 | mnt | 24130 | 23879 | − | 83 | Maintenance of lysogeny | Mnt [Enterobacteria phage P22] (NP_059641.1) | 100 | 2.E-53 |
| 43 | arc | 24158 | 24382 | + | 74 | Transcriptional repressor | Repressor arc [Escherichia coli MS 16-3] (EFU59036.1) | 99 | 8.E-46 |
| 44 | ant | 24451 | 25353 | + | 300 | Antirepressor | Ant [Enterobacteria phage P22] (NP_059643.1) | 100 | 0 |
| 45 | gp9 | 25564 | 27567 | + | 667 | Tailspike protein (Endorhamnosidase) | Tailspike protein [Enterobacteria phage P22] (AAF75060.1) | 99 | 0 |
| 46 | gtrC | 28858 | 27626 | − | 410 | O-antigen conversion; glucosyl transferase | GtrC [Enterobacteria phage P22] (YP_063719.1) | 100 | 0 |
| 47 | 28879 | 28983 | − | 34 | Unknown | ||||
| 48 | gtrB | 30050 | 29073 | − | 325 | O-antigen conversión; bactoprenol glucosyl transferase | GtrB [Enterobacteria phage ST64T] (NP_720276.1) | 99 | 0 |
| 49 | gtrA | 30364 | 30002 | − | 120 | O-antigen conversión; translocase (flipase) | GtrA [Enterobacteria phage P22] (NP_059583.1) | 100 | 2.E-80 |
| 50 | 30538 | 30660 | + | 40 | Unknown | ||||
| 51 | int | 31876 | 30713 | − | 387 | Integrase | Int [Enterobacteria phage P22] (NP_059584.1) | 99 | 0 |
| 52 | xis | 32103 | 31753 | − | 116 | Excisionase | Xis [Enterobacteria phage P22] (NP_059585.1) | 100 | 3.E-79 |
| 53 | eaC | 32741 | 32106 | − | 211 | Unknown | EaC [Enterobacteria phage P22] YP_063720.1 | 99 | 7.E-154 |
| 54 | eaG | 33021 | 32842 | − | 60 | Unknown | EaG [Enterobacteria phage P22] (NP_059587.1) | 100 | 4.E-34 |
| 55 | eaA | 34071 | 33118 | − | 317 | Unknown | EaA [Enterobacteria phage P22] (NP_059588.1) | 100 | 0 |
| 56 | eaI | 34269 | 34075 | − | 64 | Unknown | EaI [Enterobacteria phage P22] (NP_059589.1) | 100 | 3.E-36 |
| 57 | 35147 | 34266 | − | 293 | Unknown | ORF8 [Enterobacteria phage ST104] (YP_006364.1) | 91 | 1.E-116 | |
| 58 | 35726 | 35217 | − | 169 | Unknown | ORF9 [Enterobacteria phage ST104] (YP_006365.1) | 100 | 2.E-118 | |
| 59 | 35893 | 35723 | − | 56 | Unknown | ORF10 [Enterobacteria phage ST104] (YP_006366.1) | 100 | 7.E-33 | |
| 60 | abc2 | 36197 | 35904 | − | 97 | Anti Rec-BCD protein | Abc2 [Enterobacteria phage ST104] (YP_006367.1) | 100 | 4.E-63 |
| 61 | abc1 | 36528 | 36244 | − | 94 | Anti Rec-BCD protein | Abc1 [Enterobacteria phage ST104] (YP_006368.1) | 100 | 3.E-62 |
| 62 | erf | 37235 | 36528 | − | 235 | Recombination protein | ORF13 [Enterobacteria phage ST104] (YP_006369.1) | 100 | 1.E-173 |
| 63 | arf | 37375 | 37232 | − | 47 | Recombination protein | Arf [Enterobacteria phage P22] (NP_059597.1) | 100 | 3.E-24 |
| 64 | kil | 37553 | 37365 | − | 62 | Inhibitor of host septation | Kil [Enterobacteria phage P22] (NP_059598.1) | 100 | 2.E-38 |
| 65 | c3 | 37692 | 37534 | − | 52 | Regulatory protein | C3 [Enterobacteria phage P22] (NP_059599.1) | 100 | 2.E-29 |
| 66 | 17 | 38089 | 37778 | − | 103 | Superinfection exclusion | Hypothetical protein P22gp40 (NP_059600.1) | 100 | 9.E-70 |
| 67 | 38040 | 38159 | + | 39 | Unknown | ||||
| 68 | 38440 | 38237 | − | 67 | Unknown | Hypothetical protein P22gp41 (CAA33649.1) | 99 | 4.E-41 | |
| 69 | 38676 | 38440 | − | 78 | Unknown | Hypothetical protein P22gp42 (NP_059602.1) | 100 | 2.E-47 | |
| 70 | 38773 | 38648 | − | 41 | Unknown | Hypothetical protein ST64Tp22 (NP_720296.1) | 92 | 5.E-07 | |
| 71 | ral | 38907 | 38713 | − | 64 | Antirestriction protein | Ral [Enterobacteria phage P22] (NP_059603.1) | 100 | 2.E-37 |
| 72 | 38977 | 38891 | − | 28 | Unknown | Hypothetical protein lambdap47 (NP_040623.1) | 100 | 4.E-27 | |
| 73 | sieB | 38945 | 39700 | + | 251 | Superinfection exclusion | SieB [Enterobacteria phage P22] (NP_059604.1) | 100 | 3.E-138 |
| 74 | 24 | 40023 | 39721 | − | 100 | Antitermination protein | Hypothetical protein P22gp46 (NP_059605.1) | 100 | 3.E-65 |
| 75 | 40043 | 40255 | + | 70 | Unknown | ||||
| 76 | c2 | 41027 | 40377 | + | 216 | Prophage repressor | C2 [Enterobacteria phage P22] (NP_059606.1) | 100 | 4.E-158 |
| 77 | cro | 41108 | 41293 | + | 61 | Antirepressor | Cro [Enterobacteria phage P22] (NP_059607.1) | 100 | 2.E-35 |
| 78 | 41354 | 41241 | − | 37 | Unknown | ||||
| 79 | c1 | 41400 | 41678 | + | 92 | Transcriptional activator | C1 [Enterobacteria phage P22] (NP_059608.1) | 100 | 2.E-59 |
| 80 | 41668 | 41809 | + | 46 | Unknown | ||||
Gene numbers correspond with their predicted function, if known, followed by the nature of the evidence that supports the functional classification. Genes with no functional prediction, but with significant sequence similarity to genes in the NCBI database as determined by BLASTP are also listed.