Table 2.
Features of bacteriophage UAB_Phi78 genome, ORFs, gene products, and functional assignments.
| ORF | Gene | Position (nt) | No of amino acids | Predictive function | Closest hit (Accession number) | % Amino acid identity | Best e-value | |
|---|---|---|---|---|---|---|---|---|
| From | To | |||||||
| 1 | 662 | 949 | 95 | Unknown | ||||
| 2 | 954 | 1016 | 20 | Unknown | ||||
| 3 | 1062 | 1241 | 59 | Unknown | ||||
| 4 | 1238 | 1417 | 59 | Unknown | Gp3 Bacteriophage SP6 (AAP48742.1) | 88 | 4.E-28 | |
| 5 | 1410 | 1619 | 69 | Unknown | Gp4 [Bacteriophage SP6] (AAP48743.1) | 76 | 5.E-25 | |
| 6 | 1773 | 2129 | 118 | Unknown | Gp5 [Bacteriophage SP6] (AAP48744.1) | 75 | 3.E-36 | |
| 7 | 2130 | 2249 | 39 | Unknown | ||||
| 8 | 2319 | 2474 | 51 | Unknown | ||||
| 9 | 2537 | 3412 | 291 | Unknown | Gp6 [Bacteriophage K1E] (CAJ29406.1) | 72 | 2.E-142 | |
| 10 | gp8 | 3487 | 6111 | 874 | DNA-directed RNA polymerase | Gp8 [Bacteriophage SP6] (AAP48747.1) | 98 | 0 |
| 11 | 6771 | 6845 | 24 | Unknown | ||||
| 12 | 6849 | 6974 | 41 | Unknown | gene 1.1 [Bacteriophage T7] (AAP33970.1) | 65 | 0.012 | |
| 13 | gp10 | 6976 | 8871 | 631 | DNA primase | Gp10 [Bacteriophage SP6] (AF159357.1) | 98 | 0 |
| 14 | 9065 | 9469 | 134 | Unknown | ||||
| 15 | 9381 | 9614 | 77 | Unknown | ||||
| 16 | 9607 | 9807 | 66 | Unknown | Gp11 [Bacteropphage SP6] (AAP48750.1) | 89 | 2.E-34 | |
| 17 | 9758 | 10000 | 80 | Unknown | Gp12 [Bacteriophage SP6] (AAP48751.1) | 93 | 9.E-27 | |
| 18 | 10068 | 10172 | 33 | Unknown | Gp11.5 [Bacteriophage K1E] (CAJ29415.1) | 59 | 0.004 | |
| 19 | 10159 | 10377 | 72 | Unknown | Gp12 [Bacteriophage K1E] (CAJ29416.1) | 93 | 3.E-42 | |
| 20 | gp14 | 10364 | 12910 | 848 | DNA polymerase | Gp14 [Bacteriophage SP6] (AAP48753.1) | 95 | 0 |
| 21 | 12910 | 13008 | 32 | Unknown | Gp15 [Bacteriophage SP6] (AAP48754.1) | 75 | 6.E-07 | |
| 22 | 13123 | 13500 | 125 | Unknown | Gp17 [Bacteriophage SP6] (AAP48756.1) | 64 | 2.E-47 | |
| 23 | 13580 | 14389 | 269 | Unknown | Gp18 [Bacteriophage SP6] (AAP48757.1) | 92 | 6.E-180 | |
| 24 | 14407 | 14625 | 72 | Unknown | Gp19 [Bacteriophage SP6] (AAP48758.1) | 100 | 4.E-45 | |
| 25 | 14645 | 14728 | 27 | Unknown | ||||
| 26 | 14731 | 15099 | 122 | Unknown | Gp20 [Bacteriophage SP6] (AAP48759.1) | 92 | 1.E-62 | |
| 27 | 15165 | 15476 | 103 | Unknown | ||||
| 28 | gp21 | 15383 | 16414 | 343 | Exonuclease | Gp21 [Bacteriophage SP6] (AAP48760.1) | 96 | 0 |
| 29 | gp22 | 16399 | 16809 | 136 | Endonuclease | Gp22 [Bacteriophage SP6] (AAP48761.1) | 97 | 4.E-90 |
| 30 | 16895 | 17809 | 304 | Unknown | Gp23 [Bacteriophage SP6] (AAP48762.2) | 98 | 0 | |
| 31 | 17910 | 18359 | 149 | Unknown | 23 [Bacteriophage K1-5] (AAR90065.1) | 63 | 4.E-53 | |
| 32 | gp25 | 18359 | 19306 | 315 | DNA ligase | Gp25 [Bacteriophage SP6] (AAP48764.1) | 96 | 0 |
| 33 | 19278 | 19493 | 71 | Unknown | Gp26 [Bacteriophage SP6] (AAP48765.1) | 98 | 6.E-35 | |
| 34 | 19456 | 19599 | 47 | Unknown | Gp27 [Bacteriophage SP6] (AAP48766.1) | 90 | 1.E-09 | |
| 35 | 19629 | 20090 | 153 | Unknown | Gp28 [Bacteriophage SP6] (AAP48767.1) | 98 | 6.E-106 | |
| 36 | 20100 | 20309 | 69 | Unknown | Gp29 [Bacteriophage SP6] (AAP48768.1) | 99 | 9.E-38 | |
| 37 | gp30 | 20311 | 21858 | 515 | Portal protein | Gp30 [Bacteriophage SP6] (AAP48769.1) | 99 | 0 |
| 38 | gp31 | 22329 | 22706 | 125 | Scaffolding protein | Gp31 [Bacteriophage SP6] (AAP48770.1) | 92 | 2.E-71 |
| 39 | 22782 | 23078 | 98 | Unknown | ||||
| 40 | gp32 | 23256 | 24458 | 400 | Major capside protein | Gp32 [Bacteriophage SP6] (AAP48771.1) | 98 | 0 |
| 41 | gp33 | 24514 | 25254 | 246 | Tail protein | Gp33 [Bacteriophage SP6] (AAP48772.1) | 98 | 0 |
| 42 | gp34 | 25254 | 27677 | 807 | Tail protein | Gp34 [Bacteriophage SP6] (AAP48773.1) | 98 | 0 |
| 43 | gp35 | 27668 | 28387 | 239 | Internal virion protein | Gp35 [Bacteriophage SP6] (AAP48774.1) | 98 | 1.E-165 |
| 44 | 28388 | 31324 | 978 | Unknown | Gp36 [Bacteriophage SP6] (AAP48775.1) | 99 | 0 | |
| 45 | gp37 | 31391 | 35203 | 1270 | Internal virion protein | Gp37 [Bacteriophage SP6] (AAP48776.1) | 99 | 0 |
| 46 | gp38 | 35203 | 36162 | 319 | Tail fiber protein (adaptor) | Gp38 [Bacteriophage SP6] (AAP48777.1) | 98 | 0 |
| 47 | gp39 | 36171 | 36365 | 64 | Putative holin | Gp39 [Bacteriophage SP6] (AAP48778.1) | 97 | 2.E-35 |
| 48 | gp40 | 36505 | 36651 | 48 | Terminase (small subunit) | Gp40 [Bacteriophage SP6] (AAP48779.1) | 96 | 4.E-23 |
| 49 | gp41 | 36651 | 38549 | 632 | Terminase (large subunit) | Gp41 [Bacteriophage SP6] (AAP48780.1) | 97 | 0 |
| 50 | 38702 | 38977 | 91 | Unknown | Gp41 [Bacteriophage K1E] (CAJ29452.1) | 76 | 2.E-40 | |
| 51 | gp42 | 38993 | 39280 | 95 | Putative Acetyl-CoA acetyltransferase | Gp42 [Bacteriophage K1E] (CAJ29453.1) | 83 | 8.E-47 |
| 52 | gp46 | 39283 | 39627 | 114 | Peptidase_M15_3 | Gp46 [Bacteriophage SP6] (AAP48785.1) | 75 | 4.E-60 |
| 53 | 39627 | 39764 | 45 | Unknown | ||||
| 54 | 39981 | 40166 | 61 | Unknown | Gp45 [Bacteriophage K1E] (CAJ29456.1) | 88 | 6.E-30 | |
| 55 | gp49 | 40297 | 41949 | 550 | Tail spike protein | Gp49 [Bacteriophage SP6] (AAP48788.1) | 98 | 0 |
| 56 | 42033 | 43547 | 504 | Unknown | Gp50 [Bacteriophage SP6] (AAP48789.1) | 96 | 0 | |
| 57 | 43555 | 43698 | 47 | Unknown | Gp51 [Bacteriophage SP6] (AAP48790.1) | 96 | 3.E-22 | |
| 58 | 43752 | 43829 | 25 | Unknown | Gp52 [Bacteriophage SP6] (AAP48791.2) | 100 | 3.E-19 | |
Gene numbers correspond with their predicted function, if known, followed by the nature of the evidence that supports the functional classification. Genes with no functional prediction, but with significant sequence similarity to genes in the NCBI database as determined by BLASTP are also listed.