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. 2016 Apr;5(2):167–175. doi: 10.21037/tau.2016.02.03

Figure 2.

Figure 2

Time response of JAK/STAT pathway activation in RT4-D6P2T after BDNF administration. BDNF (100 pM) was administered to treat rat Schwann cells RT4-D6P2T at 0 min, 10 min, 30 min, 60 min, 120 min and 24 hr. STAT3/STAT1 were activated at 10 min after the treatment with BDNF (lane 2), and the phosphorylation level peaked at 30 min (lane 3) (*P<0.01). One hour later, the pSTAT3/pSTAT1 returned to baseline level (lane 4). Interestingly, STAT3/STAT1 regained phosphorylation at 2 hr post BDNF treatment (lane 5) and reached a second peak at 24 hr, which was even higher than the first peak (lane 6) (#P<0.01). (A) Panel represents western blotting and (B) panel represents the phosphorylation ratio of STAT1 and STAT3 in RT4-D6P2T. Four independent experiments were done for each data point, and the error bars represent ± SD. JAK, Janus kinase; STAT, signal transducer and activator of transcription; BDNF, brain-derived neurotrophic factor.