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. 2016 Apr;5(2):167–175. doi: 10.21037/tau.2016.02.03

Figure 3.

Figure 3

Time response of JAK/STAT pathway activation in HSCs after BDNF treatment. BDNF (100 pM) was administered to treat the human Schwann cells at 0 min, 10 min, 30 min, 60 min, 120 min, 24 hr and 72 hr. STAT3/STAT1 were activated at 10 min after the treatment with BDNF (lane 2), and the phosphorylation level peaked at 60 min (lane 4) (*P<0.01). In human Schwann cells, STAT3/STAT1 phosphorylation sustained for a relatively longer response period than in rat Schwann cells. Two hours later, the pSTAT1 returned to near baseline level (lane 5), while the pSTAT3 remained at a higher than baseline level. In addition, STAT3/STAT1 regained phosphorylation and reached a second peak at 24–48 hr (lane 6&7) (#P<0.01). The activation of STAT1 was totally quenched at 72 hr post-BDNF treatment (lane 8), while pSTAT3 remained at a higher than baseline level also. (A) Panel represents western blotting and (B) panel represents the phosphorylation ratio of STAT1 and STAT3 in HSCs. Three independent experiments were done for each data point, and the error bars represent ± SD. JAK, Janus kinase; STAT, signal transducer and activator of transcription; HSCs, human Schwann cells; BDNF, brain-derived neurotrophic factor.