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. Author manuscript; available in PMC: 2016 Apr 20.
Published in final edited form as: Cell Rep. 2015 Sep 3;12(11):1723–1730. doi: 10.1016/j.celrep.2015.08.017

Figure 1. Drosophila Fgn Knockdown Increases Synaptic Connections In Vivo.

Figure 1

(A–D) Confocal images of third instar larval NMJs, muscles 6 and 7, labeled with α-HRP (white) to detect presynaptic neuronal membranes. The following are shown: (A) a Ctl NMJ; (B) an NMJ from an Fgn-knockdown animal (Dcr2;UAS:Fgn-RNAi;elav-Gal4; note the significant increase in bouton number in Fgn-knockdown animals compared to Ctls); (C) a representative individual bouton from Ctl animals; (D) a bouton with associated satellite boutons from an Fgn-knockdown animal. Arrows show the bouton; arrowheads show satellites. Scale bar represents 10 μm.

(E) Quantification of the number of total boutons and satellite boutons in Fgn-knockdown animals compared to Ctls is shown.

(F) Quantification of IF intensity of acetylated tubulin (top), total tubulin (middle), and ratio of acetylated to total tubulin (bottom) between Fgn-knockdown animals (Dcr2;UAS:Fgn-RNAi;elav-Gal4) and outcrossed Ctls (Dcr2;;elav-Gal4 and UAS:Fgn-RNAi/+) at both the axonal shaft and distal synapse near the bouton. Note the significant increase of the ratio of acetylated tubulin to total tubulin. Error bars represent SE. *p < 0.05, ***p < 0.001.