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. 2016 Apr 20;6:24606. doi: 10.1038/srep24606

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Copyright © 2016, Macmillan Publishers Limited

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(A) Graphic representation of cell growth rates by T47D cells stably expressing Twist or control vector. Cell counts were obtained daily over a 4 day period. Presented data are the mean ± SD from two independent experiments with triplicate samples. NS stands for statistically non-significant. (B) Tumorsphere formation was assessed in T47D cells overexpressing Twist under normoxic or hypoxic conditions. Representative images of tumorspheres are shown in the right panel. Scale bars, 100 μm. Left panel, are graphic representations of tumorsphere number. Presented data are the percentage of control vector values, with mean ± SD of three separate experiments performed in duplicate. ^#p < 0.05 and *p < 0.01 when vector control cells compared with their Twist-expressing clones, respectively.