Figure 4. Overexpression of Twist enhances cell migration and invasion of T47D cells.

(A) Graphic representation of the migratory capability of stably transfected T47D cells expressing either Twist or control vector assessed using a wound healing assay. A scratch (“wound”) was inflicted to a cell layer produced 48 hours post-plating, and culture continued for an additional 24 hrs. Wound closures were photographed at 0 and 24 hr. Presented data are the mean ± SD from three independent experiments, with *indicates p < 0.01 when comparing with control values. A representative experiment is shown in the right panel. Scale bars, 50 μm. (B) Graphic representation of the invasiveness of T47D cells stably expressing Twist or control vector using a modified Boyden Chamber invasion assay as described in the Materials and Methods. Presented data are the mean ± SD from three separate experiments, with *indicates p < 0.01 when comparing with control values. A representative experiment is shown in the right panel. Scale bars, 100 μm.