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. 2016 Apr 20;6:24606. doi: 10.1038/srep24606

Figure 5. Overexpression of Twist induces the activation of PAR1 signaling.

Figure 5

(A) Graphic representation of the fold change in mRNA levels of Twist, F2R and F2RL2 in Twist-expressing T47D cells compared with control vector cells by real-time PCR. Presented data are the mean ± SD of three separate experiments, with *indicates p < 0.01 when comparing with control values. (B) Western blot analysis for p-TAZ, TAZ and CTGF expression in EMT-induced Twist-expressing T47D cells or T47D cells expressing control vector. Actin served as a loading control. (C) Effect of TAZ siRNA or NTC siRNA on CTGF promoter luciferase activity in Twist-overexpressing T47D cells and T47D cells expressing control vector. Assessments were made after 48 hours in culture. Presented data are mean ± SD of normalized luciferase activities determined from three separate experiments. *indicates p < 0.01 when control siRNA expressed in Twist-T47D cells compared with in vector control cells; and **indicates p < 0.01 when compared expression of TAZ siRNA and control siRNA in Twist-T47D cells. (D) Effect of TAZ siRNA on TAZ and Twist expression in EMT-induced Twist-expressing T47D cells and in T47D cells expressing control vector by western blot analysis. Actin served as a loading control.