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. 2016 Apr 20;6:24606. doi: 10.1038/srep24606

Figure 6. Knockdown of TAZ suppresses Twist-induced cell migration and invasion of T47D cells.

Figure 6

(A) Effect of TAZ siRNA on cell migrating activity in Twist-overexpressing T47D cells and T47D cells expressing control vector using a wound healing assay. A scratch (“wound”) was inflicted to a cell layer produced 48 hours post-plating, and culture continued for an additional 24 hrs. Wound closures were photographed at 0 and 24 hr. Presented data are the mean ± SD from three independent experiments, with *and #indicating significant difference of p < 0.05 from control values. (B) Effect of TAZ siRNA on cell invasiveness in Twist-overexpressing T47D cells and T47D cells expressing control vector using a modified Boyden Chamber invasion assay as described in the Materials and Methods. Presented data are a graphic representation of the mean ± SD of percentage of invasive cells obtained from three separate experiments, with *and #indicating significant difference of p < 0.05 from control values. (C) Expression of Twist results in increased expression of PAR1, which promotes invasion, migration, and induces CSC-like properties in breast cancer cells by upregulating the expression of TAZ.