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. 2016 Apr 20;6:24750. doi: 10.1038/srep24750

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Copyright © 2016, Macmillan Publishers Limited

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Aminoacylation efficiencies were determined from initial rates and calculated as pmol/min of aminoacylated tRNA^Trp synthesized during a 1 min incubation. The assays included 200 nM TrpRS and 500 μM yeast tRNA. Values represent the means ± standard deviation from four experiments. (A) Aminoacylation activity of human mini WT, K114Q, K153Q, K418Q, and E451Q TrpRSs. (B) Aminoacylation activity of zebrafish mini WT, Q107K, Q146K, Q411K, and H445E TrpRSs.