Figure 2. Recombineering using room temperature electrocompetent cells.

(a) Colony number of a standard LLHR assay¹⁷ in GB05-dir from the normal and the cold method in E. coli. (b) As for A, but with pBR322 origin. (c) As for A, but with a standard LCHR assay in GB05-red. (d) The electrocompetent cells were prepared on ice first. After adding PCR product kan cassette into the ice-cold electrocompetent cells, the cells plus DNA mixture were shifted to RT for 3 minutes before electroporation (middle column). CT, Cold temperature; RT, Room temperature. Error bars, SD; n = 3.