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. 2016 Mar 30;6:23763. doi: 10.1038/srep23763

Figure 2. Detection of the FRET phenomenon using the acceptor photobleaching method in UGT1A-CFP and UGT1A-YFP co-infected cells.

Figure 2

Sf9 cells were co-infected with recombinant CFP and YFP Baculovirus (negative control, A) and no increase in CFP fluorescence was observed in the circle zones bleached by the 515 nm laser line. In CFP-linker-YFP (positive control, B) UGT1A1*1-CFP + UGT1A1*1-YFP (C) and UGT1A1*9-CFP + UGT1A1*9-YFP (D)-infected cells, an increase in CFP fluorescence was observed in the circle zones bleached by the 515 nm laser line indicating oligomerization between UGT1A1 or UGT1A9 allozymes.