Table 1. Overview of 28 LIN28B-targeting miRNAs, categorized according to LIN28B-targeting potential in primary neuroblastoma tumors.
LIN28B-targeting miRNAs are filtered for their relevance in neuroblastoma according to significant negative correlation to LIN28B expression in a neuroblastoma patient cohort. In total, 12 out of 28 miRNAs show negative correlation to LIN28B expression and are considered to be relevant for neuroblastoma, while 3 out of 28 miRNAs show positive correlation to LIN28B expression. Four miRNAs lack correlation to LIN28B expression (n.s.: not significant, Spearman correlation, Benjamini & Hochberg multiple testing corrected p-value > 0.05), and for 9 miRNAs no expression data was available (n.d.: no data available). In addition, of the 15 miRNAs showing correlation to LIN28B expression, the expression of 3 miRNAs is associated to poor outcome of stage 4 neuroblastoma patients (n = 92). n.s.: not significant (log-rank test, p-value > 0.05)
| miRNA | mirBase Accession | Reference in miRTarBase | Spearman rho | log-rank odds ratio |
|---|---|---|---|---|
| miRNA’s with negative correlation to LIN28B expression | ||||
|
| ||||
| hsa-let-7a-5p | MIMAT0000062 | −0.35 | n.s. | |
| hsa-let-7b-5p | MIMAT0000063 | [6,8] | −0.36 | n.s. |
| hsa-let-7c-5p | MIMAT0000064 | −0.23 | n.s. | |
| hsa-let-7d-5p | MIMAT0000065 | [4] | −0.59 | n.s. |
| hsa-let-7f-5p | MIMAT0000067 | −0.45 | n.s. | |
| hsa-let-7g-5p | MIMAT0000414 | −0.54 | n.s. | |
| hsa-let-7i-5p | MIMAT0000415 | −0.60 | n.s. | |
| hsa-miR-125b-5p | MIMAT0000423 | [7] | −0.25 | 14.43 |
| hsa-miR-26a-5p | MIMAT0000082 | −0.37 | 12.48 | |
| hsa-miR-26b-5p | MIMAT0000083 | −0.31 | 13.00 | |
| hsa-miR-363-3p | MIMAT0000707 | −0.23 | n.s. | |
| hsa-miR-98-5p | MIMAT0000096 | −0.49 | n.s. | |
|
| ||||
| miRNA’s with positive correlation to LIN28B expression | ||||
|
| ||||
| hsa-miR-516b-3p | MIMAT0002860 | 0.17 | n.s. | |
| hsa-miR-550a-3p | MIMAT0003257 | 0.28 | n.s. | |
| hsa-miR-641 | MIMAT0003311 | 0.29 | n.s. | |
|
| ||||
| miRNA’s without correlation to LIN28B expression | ||||
|
| ||||
| hsa-let-7e-5p | MIMAT0000066 | n.s. | – | |
| hsa-miR-125a-5p | MIMAT0000443 | [5] | n.s. | – |
| hsa-miR-367-3p | MIMAT0000719 | n.s. | – | |
| hsa-miR-383-5p | MIMAT0000738 | n.s. | – | |
|
| ||||
| miRNA’s with missing data | ||||
|
| ||||
| hsa-miR-182-3p | MIMAT0000260 | n.d. | – | |
| hsa-miR-208a-3p | MIMAT0000241 | n.d. | – | |
| hsa-miR-484 | MIMAT0002174 | [5] | n.d. | – |
| hsa-miR-499a-5p | MIMAT0002870 | n.d. | – | |
| hsa-miR-552-3p | MIMAT0003215 | n.d. | – | |
| hsa-miR-561-3p | MIMAT0003225 | n.d. | – | |
| hsa-miR-569 | MIMAT0003234 | n.d. | – | |
| hsa-miR-605-5p | MIMAT0003273 | n.d. | – | |
| hsa-miR-92b-3p | MIMAT0003218 | n.d. | – | |