Table 2.
Metabolic measures and fat depots following rAAV leptin treatment.
| Time point | GFP | LD | MD | HD |
|---|---|---|---|---|
| Change in % Fat Day 15 | −0.15 (0.33) | −0.38 (0.36) | −1.18 (0.25) | −3.31 (0.41)abc |
| Change in %Fat Day 30 | 0.04 (0.37) | −0.08 (0.48) | −1.30 (0.38)a | −2.48 (0.37)ab |
| Change in % Fat Day 45 | 0.32 (0.29) | 0.34 (0.51) | −1.11 (0.41)ab | −2.61 (0.47)abc |
| Change in % Fat Day 60 | 0.61 (0.31) | 0.65 (0.41) | −0.62 (0.59) | −1.89 (0.42)ab |
| Total adiposity (g) | 14.78 (1.64) | 15.81 (0.84) | 11.21 (2.34 | 4.96 (1.19)abc |
| UCP1 (% of control) | 100 (8.84) | 96.75 (9.15) | 114.8 (15.90) | 122.7 (12.91) |
| RQ | 0.86 (0.01) | 0.85 (0.01) | 0.86 (0.01) | 0.88 (0.01) |
Data represents the mean ± S.E.M. Change in % fat mass was calculated as the difference between the percentage of fat mass at the indicated day and the percentage of fat mass at day 0 for each individual animal. Respiratory quotient was measured at day 53. Adiposity represents the cumulative wet tissue weight of the epididymal, retroperitoneal and perirenal white adipose tissue depots. UCP1 levels for the control were set to 100 and the S.E.M. of groups adjusted accordingly. Data for change in % fat were analyzed by two-way ANOVA with Bonferroni post-tests. P<0.001 for interaction. Data for total adiposity (P<0.001), UCP1 (NS), and RQ (NS) were analyzed by one-way ANOVA with Tukey post-tests.
P<0.05 for difference from Control.
P<0.05 for difference from LD.
P<0.05 for difference from MD.
Western Blot: Top image represents UCP1 and bottom Beta-tublin.
