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. 2016 Apr 18;7:11344. doi: 10.1038/ncomms11344

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(a) portion of ¹H-¹⁵N HSQC spectrum of RXFP1_(1–72) showing chemical shift effects on titration with EL1^(475–486)/EL2-RXFP1 (b) chemical shift differences for wild-type RXFP_(1–72) (black), F54A (blue) and G41A/D42A (red) (c) wild-type RXFP_(1–72) (black), G45A/W46A (blue) and C40A^ins (red). Experiments were conducted at pH 6.8 and 25 °C.