Figure 4. Mincle-induced p38 activation is required for iNOS translation.

(a,b) WT BMDMs were stimulated with TDM or Pam3 or co-stimulated with Pam3 and TDM in the presence of the indicated chemical inhibitors, for 12 h. (a) Nitric oxide production in culture supernatants. ***P<0.001 (Student's t-test). (b) left: qRT-PCR analysis of iNOS mRNA levels from stimulated macrophages; right: immunoblot analysis of iNOS protein expression. (c) Immunoblot analysis of phospho-p38 from WT and Mincle^−/− BMDMs treated with Pam3, TDM or co-stimulated with Pam3 and TDM for 1 or 12 h. Data are representative of at least three independent experiments.